Repair and regeneration of cartilage and bone cells derived using postpartum

An ex vivo method of inducing differentiation of cells may be obtained from tissue substantially free human umbilical cord blood, where the cells are capable of self-renewal and differentiation, and wherein expression by said cells of genes encoding interleukin 8 ; reticulon 1; chemokine ligand 1 (CXC motif); chemokine ligand 3 (CXC motif); ligand 6 Chemokine (CXC motif) and protein 3 by tumor necrosis factor alpha induced is increased relative to a human cell that is a fibroblast, a stem cell mesenchymal or bone stem cell iliac crest as characterized in a set gene, a chondrogenic phenotype comprising exposing said cells to one or more chondrogenic differentiation inducing agents wherein said cells further comprise the following characteristics: (i) potential to undergo at least 40 doublings in culture; (Ii) binding and expansion or uncoated, wherein the culture vessel coated fabric comprises a coating of gelatin, laminin, collagen, polyornithine, vitronectin or fibronectin a culture vessel coated fabric; (Iii) production of vimentin and alpha smooth muscle actin; (Iv) production of each of CD10, CD13, CD44, CD73, CD90, PDGFr-alpha and HLA-A, B, C, as detected by flow cytometry; (V). lack of expression of CD31, CD34, CD45, CD80, CD86, CD117, CD141, CD178, B7-H2, HLA-G and HLA-DR, DP, DQ as detected by flow cytometry; (Vi) secretion of MCP-1, IL-6, GCP-2, IL-8, TIMP-1, TPO, KGF, HGF, FGF, HBEGF and BDNF as detected potr ELISA; (Vii) lack of secretion of SDF-1alpha, VEGF, TGF-beta2, ANG2 and PDGFBB as detected by ELISA; (Viii). growth to about 5% about 20% oxygen (ix). require L-valine for growth; (X) expression of PD-L2 and tissue factor as measured by flow cytometry; (Xi) a decrease in expression of the following genes relative to a human cell that is a fibroblast, a mesenchymal stem cell or marrow stem iliac crest cell as characterized in a set of genes: homeobox 2 short stature; 2 protein heat shock 27 kDa; ligand 12 chemokine (CXC motif) (derived factor 1 stromal cells); elastin; CDNA DKFZp586M2022 (from clone DKFZp586M2022); mesenchyme homeobox 2; homeobox homolog 1 of "sine oculis"; crystalline, alpha B; activator associated with morphogenesis 2 "disheveled"; DKFZP586B2420 protein; similar to neuralin 1; tetranectin; domain 3 src homology (SH3) and cysteine ​​rich; translocation gene 1 B, antiproliferative lymphocytes; cholesterol 25-hydroxylase; 3 transcription factor related dwarfism; FLJ23191 hypothetical protein; interleukin 11 receptor, alpha; enhancer procollagen C endopeptidase; homolog 7 "frizzled"; hypothetical gene BC008967; collagen, type VIII, alpha 1; tenascin C; homeobox protein iroquois 5; hephaestin; integrin, beta 8; synaptic vesicle glycoprotein 2; CDNA FLJ12280 fis, clone MAMMA1001744; factor 1 receptor-like cytokines; channel calcium activated potassium intermediate / low conductance, subfamily N, member 4; Integrin, alpha 7; DKFZP586L151 protein; transcription coactivator with PDZ-binding motif (TAZ); homeobox homolog 2 of "sine oculis"; KIAA1034 protein; early growth response 3; homeobox of "distal less" 5; FLJ20373 hypothetical protein; 1 family aldo-keto reductases of C3 member (3-alpha hydroxysteroid dehydrogenase, type II); biglycan; fibronectin 1; proenkephalin; integrin, beta 1 type (with EGF-like domains of repetition); cDNA clone 1968422 Euroimage; EphA3; KIAA0367 protein; natriuretic peptide receptor C / guanylate cyclase C (atrial natriuretic peptide receptor C); FLJ14054 hypothetical protein; CDNA DKFZp564B222 (from clone DKFZp564B222); protein vesicle-associated membrane 5; protein 1 fibulin type EGF containing extracellular matrix; 3 like protein interaction BCL2 / adenovirus E1B 19 kDa; binding protein 1 to AE; 1 VIIa polypeptide subunit of the cytochrome c oxidase (muscle); neuroblastoma, suppression of tumorigenicity 1; and binding protein 2 insulin- like growth factor, 36 kDa; and (xii) producing GCP-2 and NOGO-A as assayed by immunocytochemistry.