Effect of protoanemonin on expression of virulence factors regulated by quorum sensing system of Pseudomonas aeruginosa

Objective To study the effect of protoanemonin on the expression of virulence factors regulated by quorum sensing(QS) system of Pseudomonas aeruginosa(PA).Methods The minimum inhibitory concentration and growth curve of protoanemonin on PA were determined.With furanone C-30(40 μmol/L) as positive control,the effect of protoanemonin(40 and 80 μmol/L) on the growth of PAO1 was assessed,and the effects on the expression levels of the extracellular virulence factors(pyocyanin,protease,and elastase) regulated by QS system were also evaluated.Results Protoanemonin in concentration used in this study had no significant effect on the growth of PAO1,but it had the inhibitory effect on the three virulence factors of PAO1 with a dosage-dependent manner.Conclusion It is possible that protoanemonin acts on QS system of PA to inhibit the expression of virulence factors,in order to reduce the virulence of the bacteria.