A restriction fragment length polymorphism (RFLP) locus of rat (Rattus norvegicus) linked to the Cs-1 locus of rat linkage group XIII

A novel restriction fragment length polymorphism (RFLP) in inbred rats was revealed by Southern blot analysis with a clone arbitrarily chosen from a rat genomic library as a probe. A clone named α403 showed interstrain variations in the length of theEcoRI andHindIII fragments. TheEcoRI fragments were either 0.7 or 3 kb, those ofHindIII were either 4.5 or 5 kb, and three types were identified as combinations of those fragments in 20 inbred rat strains. These types segregated in backcross progeny as codominant alleles. The locus for the RFLP was thus namedA403. Analysis of linkage between the RFLP locus and 13 other loci reveal that theA403 locus was closely linked to theCs-1 locus (15 ± 5.2%), which belongs to rat linkage group XIII.