Small-form-factor LED-based light application surface for indocyanine green-mediated antibacterial photothermal therapy

We present here a small-form-factor LED-based antimicrobial photothermal therapy (aPTT) applicator surface, and its clinical feasibility as well as efficacy for administration of indocyanine green (ICG)-assisted aPTT to the dental plaque. Fifteen healthy adults were assigned to this four-day randomized study. After rinsing with ICG, 100J/cm2 of 810 nm light was applied to the aPTT-treatment area. Plaque area and gingival crevicular fluid matrix metalloproteinase-8 (MMP-8) were measured, and plaque bacteriomes before and after the study were analyzed using 16S rRNA sequencing. aPTT administration was preformed successfully and plaque-specifically with the applicator surface. Total plaque area and endpoint MMP-8 secretion were reduced on the aPTT-treatment side. aPTT reduced Streptococcus, Acinetobacteria, Capnocytophaga, and Rothia bacteria species in plaques. The applicator surface is feasible for aPTT self-administration, targets therapy to dental plaque, reduces plaque forming bacteria and exerts an anti-inflammatory effect.