Colorimetric analysis of a fluorescent device for quantitative detection of copper ions
2012
The present invention relates to a fluorescent colorimetric analysis, colorimetric analysis of a fluorescent detection device for quantification of copper ions: copper ion solution synthesized in the laboratory and rhodamine fluorescence enhancement indicator reaction it is possible, using self fluorescence ratio a developing apparatus, an indicator light to be excited by a specific wavelength 365nm. Capture fluorescent color before and after addition of the copper ion indicator electronic imaging device, such as scanners and digital cameras. Extracting color change before and after the red, green, three-channel color change blue (the RGB), corresponding thereto construct the standard curve the concentration gradient of copper ions. In the course of the test sample, the color change and the resulting sample values than the curve, such that the copper ion-selective unknown sample analyzed quantitatively. This method can detect 10-5M copper ion concentration, and can effectively eliminate interference nine kinds of ions coexist with high selectivity.
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