Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry for monitoring alkylation of beta-lactoglobulin B exposed to a series of N-substituted acrylamide monomers.
1999
Delayed-extraction matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry, in both linear and reflectron modes, has been used to examine the alkylation of bovine β-lactoglobulin-bound cysteines exposed to various molar concentrations (0.5–30 mM) of acrylamide and a number of its N-substituted monomers. These measurements were conducted at pH ∼ 9.5, and showed that at 0.5 mM all monomers (except N-acryloylaminopropanol) resulted in a measurable alkylation of at least one cysteine out of five. At higher concentrations (15 mM) all investigated monomers resulted in substantial alkylation which, for some, involved all five cysteine residues. Reflectron MALDI-TOF measurements of a number of alkylated protein digests revealed that, at low molar ratios, the alkylation site is influenced by the identity of the monomer. For example acrylamide and N,N-dimethylacrylamide attacked Cys160 as well as one of the three cysteines within the tryptic fragment [102–124], while other investigated monomers did not involve Cys160. The implications of the present data for two-dimensional (2D) gel electrophoresis, and their eventual correlation to the toxicity of the investigated monomers, are discussed. Copyright © 1999 John Wiley & Sons, Ltd.
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