Potentiation of T Lymphocyte Responses by Modulating NF - Kappa Beta Activity in Dendritic Cells

Abstract : The goal of this investigation is to determine whether modulation of NF-kappa beta activity by genetic engineering of DCs can affect DC longevity and T cell stimulatory functions. The specific aims are: 1) To determine the effect of expression of constitutively-active IK beta (I kappa beta kinase) on nuclear NF-kappa beta levels in DCs, and on the longevity of DCs. 2) To determine beta expression on in vitro and in vivo T cell priming by DCs. Results from these studies may thus lead the way for development of approaches for enhancing DC survival and function, with the goal of generating potent anti-tumor immunity.