Effect of Three-minute Cold Exposure in Cryosauna at –70°C on the Human Cellular Immune System

Cell components of the adaptive and innate immunity were investigated in essentially healthy volunteers aged 27 to 34 years. Peripheral blood was analyzed for absolute and relative counts of lymphocyte phenotypes CD3+, CD3+CD4+, CD3+CD8+, CD19+, CD3–CD16+CD56+, CD3+CD16+CD56+, CD3+CD25+, CD45RA+, CD4+CD45RA+, and monocytes and granulocytes expressing pattern recognition receptors of the Toll-like family (TLR2, TLR4, TLR6) on the cellular membrane. A single cold exposure at –70°С was found to affect cell factors of the human immune system dramatically. Very low temperatures produce different changes in adaptive and congenital components of immunity representing a complex process triggered by the stress-reaction to a short stay in air cryosauna.