Effects of ultrasound-guided transvaginal follicular aspiration on oocyte recovery and hormonal profiles before and after gnrh treatment

Abstract Endocrine changes and recovered oocytes were evaluated during 16 wk of ultrasound-guided transvaginal follicular aspiration (TVFA) and prior to and following administration of GnRH at the cessation of aspiration. Nonlactating previously aspirated (PAC, n = 4) and non-aspirated, (AC, n = 4) Holstein cows were subjected to 16 wk of twice-weekly aspiration. Four control cows (OAC) were aspirated 1 tune only at the final TVFA session (wk 16). Jugular blood samples were collected from all cows during aspiration, before and after the final TVFA session, and during an 18-d period following cessation of aspiration. Ovarian activity was monitored in all cows after cessation of aspiration for 18 d. The PAC and AC cows averaged 3.4 ± 1.2 (± SE) and 6.8 ± 1.2 oocytes per session, respectively. Progesterone concentrations during TVFA did not differ between the PAC and AC (0.8 ± 0.1 and 0.9 ± 0.1 ng/mL, respectively). Progesterone concentration in OAC was 4.5 ± 0.2 ng/mL before TVFA, while the PAC and AC averaged 0.5 ± 0.2 and 0.3 ± 0.2 ng/mL, respectively, at 16 wk. At Week 16 LH was 1.0 ± 0.2 ng/mL and it increased to 7.5 ± 0.1 ng/mL after GnRH treatment. The LH concentration before the final aspiration session was higher at peak amplitude in PAC than in AC groups and peak length was longer in OAC than in AC cows (P