Validation Study on Five Cytotoxicity Assays by JSAAE II. Statistical Analysis

The validation study organized by JSAAE (JSAAE study) brought to light three main statistical problems, namely, (1) how to obtain a reliable estimate of ED50 from each experiment, (2) how to check the availability of the ED50 estimate obtained from each experiment, and (3) how to evaluate the feasibility of assays included in the JSAAE study. To resolve the first problem, the authors devised a computer program (LAPJSAAE) on SAS which incorporated a non-linear least squares method to obtain estimates of ED50 based on a logistic model for raw measurements. To resolve the second problem, the authors set several criteria for checking data which had previously been treated with manual adjustments for trivial errors such as de