The method of ELISA detection fusion albumen rMBP NAP

The invention discloses a kind of method of ELISA detection fusion albumen rMBP NAP, including being coated, close, be loaded, plus Mus MBP monoclonal antibody, plus ELIAS secondary antibody, add substrate colour developing.The present invention is coated using rabbit NAP polyclonal antibody, especially through NAP GST affinitive layer purification antibody, sandwich indirect elisa method by using the ELIAS secondary antibody of commercialization, avoid the trouble preparing enzyme labelled antibody, increased sensitivity, when measuring without through special handling, simple and efficient, sensitive, specificity is good.In addition, under the working concentration of the present invention, also well quantitative analyses can be carried out to rMBP NAP：Variation within batch coefficient is less than 10%, and interassay coefficient of variation is respectively less than 15%；After adding the chaff interference NAP GST that structure is similar to, OD value does not have rising, and T testing identity does not occur significant difference；The response rate is also between 85 115%.