Количественное определение субстанции ангипур в плазме крови методом ВЭЖХ с флуоресцентной детекцией

Results of the development and validation of a method using HPLC with fluorescence detection for the quantitative determination of xanthine derivative, 3-methyl-8-(piperazine-1-yl)-7-(thietane-3-yl)-1-ethyl-1 H -purine-2,6(3 H ,7 H )-dione hydrochloride (Angipur) in plasma of blood donors are presented. Optimum conditions for the quantitative determination of Angipur substance are as follows: buffer system of 50 mm single-substituted potassium phosphate pH 6.5; ratio of the aqueous and organic phases 55:45 v/v; addition of a phase modifier sodium salt of heptanesulfonic acid (0.15%); temperature 40°C; detector extinction wavelength 290 nm; emission wavelength 340 nm. Sensitivity of the method (limit of detection) for Angipur is 2.5 ng/mL and the limit of quantitation is 5 ng/mL. The average measurement error does not exceed 15%.