EVALUATING THE ANTIPROLIFERATIVE POTENTIAL OF METHONOLIC LEAF EXTRACT OF CASSIA NIGRICANS Original Article

Objective: It is well established that plants have always been useful source as anticancer compounds. This study was attempted to investigate the in vitro anti-cancer potential of methonolic extract of Cassia nigricans on breast cancer MCF-7 cell lines. Methods: The methanol extract of C. nigricans was screened for its anti-proliferative effect against MCF-7 (Breast cancer) cell lines using MCF-7 cells seeded 96 well plates. Results: Extract was exposed with MCF-7 cell lines for 24h and 72h at a range of increasing concentrations (0-500μg/ml) in order to obtain a doseresponse graph and IC50 value. The C. nigricans extract showed cytotoxic effect in MCF-7cells with IC 50 Conclusion: The C. nigricans extract showed effective cytotoxic activity in a dose and time dependent manner. Future work will be interesting to know the chemical composition and also better understand the mechanism of action present in the extract for developing it as drug for therapeutic application. of 82.6μg/ml.