Conformational and temperature effects on separation of stereoisomers of a C3,C4-substituted β-lactamic cholesterol absorption inhibitor on amylose-based chiral stationary phases
2001
A direct liquid chromatography method was developed for the diastereo- and enantioselective analysis of a C3,C4-substituted β-lactamic hypolipodemic agent (SCH 48461) and its stereoisomers on two commercially available amylose-based chiral stationary phases (CSPs), namely, Chiralpak AS and Chiralpak AD. The mobile phase composition (type and content of alcoholic modifier) was considered to achieve baseline resolutions in a single chromatographic run. In order to investigate the influence of molecular flexibility on chiral recognition process, β-lactams were ring-opened and converted into β-amino esters derivatives. Thermodynamic parameters associated with adsorption equilibria between acyclic and cyclic stereoisomers and CSPs were calculated from chromatographic runs at various temperatures.
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