The evaluation of one tobacco articles in vitro toxicology carries out the method for cell culture with the preparation of three-dimensional cell support and using it

The one tobacco articles in vitro toxicology evaluation preparation method of three-dimensional cell support, is characterized in：It is polymerize in Tissue Culture Dish by High Internal Phase Emulsion, obtaining one kind has polymer intercommunication porous material, and material has tobacco product in vitro toxicology evaluation cell（Human lung carcinoma cell, human bronchial epithelial cell）The macropore of size, and between Kong Yukong it is intercommunication, beneficial to oxygen needed for cell growth, the input of nutriment and the output of cell metabolism waste, material surface is modified and makes it have good cellular affinity, compared with existing toxicological evaluation generally uses flat board monolayer cell culture method, cell can the present invention support on three dimensional growth, solid shape and biochemical and feature during with substance are more nearly, therefore, when carrying out the evaluation of tobacco product in vitro toxicology using the cell of the three-dimensional cell support culture of the present invention, evaluation result will be more objective, truly.It is more convenient to subsequent experimental and the cytoskeleton is prepared in culture dish.