[Protective effect of lipoxin A4 against rhabdomyolysis-induced acute kidney injury in rats].

AIM: To investigate the protective effect of lipoxin A4 (LXA(4);) against rhabdomyolysis-induced acute kidney injury (AKI) and the possible mechanism. METHODS: The animal model of AKI was produced by intramuscular injection with glycerol. SD rats were randomly divided into 3 groups: the normal, AKI and LXA(4); groups. Blood urea nitrogen and serum creatinine levels were evaluated using a HITACHI 747 automatic analyzer. For histological examination, sections were stained with HE. The activity of myeloperoxidase (MPO) was detected by spectrophotometer. Expressions of TNF-α, IL-6 were determined by ELISA, and NF-κB in renal tissues was detected by Western blot analysis. RESULTS: The levels of blood urea nitrogen and serum creatinine decreased, and the injury of kidney was improved significantly in rats treated with LXA(4); as compared with AKI rats (P<0.05). The levels of TNF-α and IL-6, the activity of MPO and the expression of NF-κB were significantly lower in LXA(4); group than in AKI group (P<0.05). CONCLUSION: LXA(4); can significantly protect rats against AKI partly by blocking NF-κB activation and reducing inflammatory cytokine expression and inflammatory cells infiltration in the kidney.