Effects of immunosuppressive agent on mRNA expression of anti-aspergillus infection-associated receptors on alveolar macrophage

Objective To investigate the effects of immunosuppressive agent on mRNA expression of anti-aspergillus infection-associated receptors on alveolar macrophage.Methods Thirty Kunming mice were randomly divided into two groups:normal control group(n=6)and immunocompromise group(150 mg/kg cyclophosphamide,intraperitoneal injection,n=24).Six mice were randomly taken to collect alveolar macrophage by bronchoalveolar lavage from immunocompromise group at time of 4,8,12 and 24 hours after immunosuppression.Mice in normal control group were sacrificed to collect alveolar macrophage after receiving normal saline intraperitoneal injection.Reverse transcription-polymerase chain reaction was used for determining the mRNA expression of Toll-like receptor 2(TLR2),TLR4 and dendritic cell-associated C-type lectin 1(Dectine-1).Results TLR2 mRNA expression decreased signiffieantly four hours after cyclophosphamide intraperitoneal injection,and decreased continually to 24 hours(all P＜0.01).The decrease of TLR4 mRNA expression appeared eight hours after immunosuppression,and decreased continually to 24 hours(all P＜0.0 1).There was not significant difference on Dectine-1 mRNA expression between normal control group and immunocompromise group.Conclusions The results suggest that cyclophosphamide could down-regulate the mRNA expression of TLR2 and TLR4 on alveolar maerophage. Key words: Immnosupression; Cyclophoaphamid; Toll-like receptor 2; Toll-like receptor 4; Dendritic cell-associated C-type lectin 1