ANÁLISE COMPARATIVA ENTRE ELFA ELISA UTILIZANDO PROTEÍNA RECOMBINANTE DE BACTERIÓFAGO E OUTROS MÉTODOS DIAGNÓSTICOS PARA DETECÇÃO DE Salmonella spp. EM PRODUTOS DE ORIGEM ANIMAL (Comparative analysis between ELFA ELISA using a recombinant bacteriophage protein and other diagnostic methods for Salmonella spp. detection in products of animal origin)

The analysis methods for Salmonella spp. detection are of great importance in the system of animal products processing industries, because they reflect directly on the microbiological quality of food, impacting the storage time and therefore the release of the product for sale. This study verified the efficiency of a new ELFA ELISA test using a recombinant protein from bacteriophage (VIDAS UP ® ) to detect the presence of Salmonella spp. in products of animal origin. Two hundred fifteen samples (15 samples artificially contaminated and 200 samples from agribusiness routine), from different products of animal origin were analyzed. The samples were tested by five methods of diagnosis: VIDAS UP ® , conventional methodology in accordance with ISO 6579, polymerase chain reaction (PCR), semi-solid Rappaport-Vassiliadis Modified (MSRV) and MSRV medium + supplement. Eleven out of 215 (5.12%) samples were positive in at least one test, which four samples were from agribusiness routine and seven were from artificially contaminated samples. The sensitivity and specificity of the VIDAS UP ® when compared with the conventional method was 90.0% and 99.51% respectively. The VIDAS UP ® was equivalent to the other methodologies, showing to be an effective and a rapid method to detect Salmonella spp. in different products of animal origin.