德氏假单胞菌(Pseudomonas delafieldii)R-8脱硫基因启动子的克隆与鉴定

The dsz promoter serial deletion fragments were cloned by PCR from strain Pseudomonas delafieldii R-8 which can convert dibenzothiophene into 2-hydroxybiphenyl and sulfate. Series fragments were cloned into the detection of expression vector pPR9TT, then reintroduced into strain R-8 to obtain engineering strains R-8-P, and the expression of reporter gene, LacZ, in R-8-P was determined. The results showed that the size of the dsz core promoter region was 300bp, and regulation region was forecasted.