[The mechanism of alteronol inhibiting the proliferation of human promyelocytic leukemia HL-60 cells].

This study is to investigate the mechanism of human promyelocytic leukemia HL-60 cells proliferation induced by alteronol in vitro.Human promyelocytic leukemia HL-60 cells cultured in vitro were treated with different concentrations of alteronol.Inhibition rate was detected by SRB assay.Cellular morphological changes were observed by Hoechst and AO/EB(acridine orange/ethidium bromide dye) staining.The apoptosis rate was determined by Annexin V-FITC/PI assay.Cell cycle distribution was determined by flow cytometry.Western blotting analysis was carried out to determine the cell cycle related proteins.The proliferation of HL-60 cells treated with alteronol was inhibited in a concentration-dependent manner.Based on cell viability assay,observation on cell morphology and apoptosis rate,it confirmed that alteronol played an obvious role in proliferation inhibition of human promyelocytic leukemia HL-60 cells,but it did not induce apoptosis in human promyelocytic leukemia HL-60 cells in different concentrations groups.Alteronol could effectively inhibit the proliferation of human promyelocytic leukemia HL-60 cells inducing cell cycle arrest at G1 phase,as well as,alteration expression of cell cycle proteins level of CyclinD1 and pRb.