High Efficiency Transformation of Bacillus brevis by Electroporation

Various conditions in the Bacillus brevis transformation by electroporation were investigated to increase the efficiency. Competent cell volume and presence of polyethylene glycol 6000 and single stranded DNA on pulsing were critical for the efficiency. As a result, we established the improved method by which 107–108 transformants/μg plasmid DNA could be obtained.