Simultaneous Derivative Spectrophotometric Estimation of Aceclofenac and Tramadol with Paracetamol in Combination Solid Dosage Forms

A derivative spectrophotometric procedure has been developed for the simultaneous determination of individual combination of aceclofenac and tramadol with paracetamol in combined tablet preparation. Tablet extracts of the drugs were prepared in distilled water. The zero crossing point technique and the compensation technique were used to estimate the amount of each drug in the combined formulations, and were compared. The results were found to be accurate and free from interferences. The procedure is rapid, simple, nondestructive, and does not require solutions of equations. Calibration graphs are linear (r=0.9999), with a zero intercept up to 24 mg/ml of each drug in combination with paracetamol. Detection limits at the p = 0.05 level of significance were calculated to be 0.5 mg/ml of aceclofenac, tramadol and paracetamol respectively.