CCR7 and its related molecules may be potential biomarkers of pulmonary arterial hypertension

Pulmonary arterial hypertension (PAH) is a chronic progressive cardiovascular disease characterized by vascular remodeling and leading to right heart failure. The purpose of this research was to further study the pathogenesis of PAH and to detect potential prognostic signatures. Differentially expressed genes (DEGs) selected from GSE38267 were mostly enriched in inflammation-related pathways, suggesting inflammation may be involved in the occurrence and development of PAH. Through the prediction and verification of related miRNAs and lncRNAs using online databases and GEO datasets, CCR7 and its related molecules, including hsa-let-7e-5p and SNHG12, were identified as possible targets. The expression levels of CCR7, hsa-let-7e-5p and SNHG12 were then verified by qRT-PCR in vivo and in vitro. Further study showed that silencing of SNHG12 decreased the expression of CCR7 under hypoxia treatment in vitro. Dual-luciferase reporter assays were utilized to verify the relationship between hsa-let-7e-5p and SNHG12. Collectively, our research reveals that a lncRNA/miRNA/mRNA interaction network may be involved in the pathogenesis of PAH, and suggest SNHG12, hsa-let-7e-5p and CCR7 as potential biomarkers for PAH.