[Influence of angiotensin-(1-7) on cell activation in rat renal interstitial fibroblasts induced by aldosterone].

AIM: To explore the influence of angiotensin-(1-7) [Ang-(1-7)] on cell activation and extracellular matrix secretion in rat renal interstitial fibroblasts(NRK-49F) induced by aldosterone(ALD).METHODS: The NRK-49F cells were cultured in vitro,and then were divided into control group,ALD group,Ang-(1-7) group,and ALD+Ang-(1-7) group.When the cells were cultured for 48 h,the expression of α-smooth muscle actin(α-SMA)(a sign of cell activation) was detected by immunocytochemistry;the level of collagen type Ⅰ(ColⅠ) in the cultured supernatant was measured by enzyme-linked immunosorbent assay(ELISA).When the cells were cultured for 30 min,the expressions of phosphorylated and total ERK1/2(pERK1/2,tERK1/2) in the cell lysate were detected by Western blotting.RESULTS: Compared with control group,the expressions of α-SMA,ColⅠ and the Phos/Total ERK1/2 ratio in ALD group and ALD+Ang-(1-7) group increased significantly(P0.05).Compared with ALD group,the expressions of α-SMA,ColⅠ and the Phos/Total ERK1/2 ratio in ALD+Ang-(1-7) group decreased significantly(P0.05).CONCLUSION: Ang-(1-7) can inhibit ALD-induced cell activation and decrease the secretion of ColⅠ in rat renal interstitial fibroblasts.Inhibition of ERK1/2 pathway may play an important role in this process.