Investigation of in vitro antileishmanial activity of moxifloxacin, linezolid and caspofungin on Leishmania tropica promastigotes.

Objective: This study aimed to evaluate the potential in vitro anti-leishmanial activities of moxifloxacin, linezolid and caspofungin against Leishmania tropica. Methods: In vitro effects of all agents were studied by using the microdilution method. For this purpose, serial dilutions of the aforementioned agents were prepared in concentrations between 4096 μg/mL-0.008 μg/mL. Afterwards, promastigotes incubated in suitable medium were counted with the hemocytometer and adjusted as having a last concentration of 2.5x106 cells/mL in wells containing medium+antibiotic or antifungal. After incubation live promastigotes were counted with the hemocytometer and inhibitor concentrations (IC50) were determined by comparing with the control that contained no antibiotics or antifungal. Results: IC50 values of moxiloxacin, linezolid and caspofungin were found as 194.7 μg/mL, 896 μg/mL and 235.7 μg/mL, respectively. Conclusion: As a result, moxifloxacin was found to be effective in lower concentrations than the other studied agents against L. tropica promastigotes. (Turkiye Parazitol Derg 2013; 37: 1-3)