Method for preparing rhNGF mature peptide

The invention discloses a method for preparing an rhNGF mature peptide. The method comprises the following steps: constructing an expression vector and engineering bacteria; expressing an inclusion body, collecting thallus, splitting, washing, dissolving and renaturating the inclusion body, and obtaining a pro-rhNGF renaturation solution; preliminarily purifying the pro-rhNGF renaturation solution through cation exchange chromatography; performing secondary purification on pro-rhNGF through hydrophobic interaction chromatography; performing trypsin enzyme digestion to remove a pro-rhNGF leading peptide, and obtaining the rhNGF mature peptide; performing third-time purification on the rhNGF mature peptide to obtain the purified rhNGF mature peptide. According to the method, industrial production of the rhNGF mature peptide can be performed, and the mature peptide is high in purity and high in yield.