Use of metabolomic profiling to identify potential markers and mechanism for bladder cancer progression.

250 Background: Bladder cancer (BCa) is the second most prevalent urological malignancy and the fourth highest cause of cancer-related death in the United States that is known to be caused by defects in xenobiotic metabolism, which is not well understood. Using unbiased mass spectrometry we report the metabolomic profiles in BCa tissues and demonstrate its biomarker potential as well reveal a possible mechanism regulating altered xenobiotic metabolism in these tumors. Methods: Total metabolome from clinically annotated bladder-derived tissues were examined using mass spectrometry coupled to reverse and aqueous normal phase separation of compounds. Class- specific metabolites were examined in urine specimens for their biomarker potential as well as analyzed using Oncomine Concept Map for alterations in bioprocesses. The latter was validated using a collection of molecular techniques like Q-PCR, immunoblot analysis, methylation assays and use of methyl transferase inhibitor, on bladder-derived cell lines an...