Determination of carbohydrates in tobacco by ultra performance liquid chromatography and tobacco cluster analysis

A new method for the determination of seven carbohydrates(rhamnose,xylose,fructose,mannose,glucose,sucrose,maltose) in tobacco by Ultra Performance Liquid Chromatography with an evaporative light scattering detector is studied.The carbohydrates were separated on an Acquity UPLC BEH Amide column by using acetonitrile-water(0.2%TEA) as the mobile phase.Recoveries of the method for the seven carbohydrates were from 85.2 % to 105.3 % with relative standard deviations(RSD)(n=6) of 0.78%～5.7 %.This method can be applied to the determination of carbohydrates in tobacco with good results.The contents of seven carbohydrates in 30 tobacco samples were determined,and the data were processed with cluster analysis.The results showed that the cluster analysis could be used in the comparison and classification of different kinds of tobacco.