Tratamiento de quemaduras con películas obtenidas por radiación gamma que contienen extracto hidroalcohólico de tara (Caesalpinia spinosa) en animales de experimentación

Objetivo. Evaluar el efecto cicatrizante de hidrogeles obtenidos con radiacion gamma embebidos con extracto hidroalcoholico de tara (Caesalpinia spinosa). Material y metodos. Se fabricaron hidrogeles de quitosano-alcohol polivinilico embebidos en extracto hidroalcoholico de tara (Q-APVEHAT), que se aplicaron sobre quemaduras en piel de conejas, para evaluar su capacidad cicatrizante frente al hidrogel solo, al extracto solo y nitrofural. La cicatrizacion de las heridas fue evaluada macroscopicamente y microscopicamente (biopsias) y con la medicion del area y el porcentaje de retraccion de la quemadura. La evaluacion se realizo a los 7, 14 y 21 dias posquemadura. Resultados. El dia 7 de tratamiento se aprecio diferencia estadisticamente significativa para el area de las heridas de los grupos (p =0,002). El area de las heridas tratadas con Q-APV-EHAT fue significativamente menor que la tratada con nitrofural (p = 0,001). Para el dia 14, no hubo diferencia estadisticamente significativa entre los tratamientos (p = 0,063). Para el dia 21 existio diferencia estadisticamente significativa entre tratamientos (p = 0,005), la piel tratada con Q-APV-EHAT tuvo significativamente menor area que la tratada connitrofural (p = 0,005) y la piel tratada con extracto de tara, significativamente menor area que la tratada con nitrofural (p = 0,033). La mayor retraccion de las heridas, confirmada por microscopia se obtuvo con el Q-APV-EHAT tanto a los 7, 14 y 21 dias de tratamiento. Conclusion. El hidrogel de quitosano-alcohol polivinilico embebido en extracto hidroalcoholico de tara mostro significativamente mayor actividad cicatrizante que el extracto hidroalcoholico de tara solo, las peliculas de quitosano (sin extracto de tara) y el nitrofural. Objective. Evaluate the healing effect of hydrogels obtained by gamma radiation imbibed with hydroalcoholic extract of Caesalpinia spinosa, aetaraAE in experimental animals. Material and methods. Experimental study. Hydroalcoholic extracts, chitosan-poly (vinyl alcohol) hydrogels films imbibed with hydroalcoholic extract of C. spinosa (HA-Q-CS) were made. This hydrogel was applied on burns in female rabbitAEs skin to evaluate its healing effects versus hydrogel alone, C. spinosa extract and nitrofural. Healing of wounds was evaluated, macroscopically a microscopically (biopsies) as well the evaluation of the area and percent of burn retraction. The evaluations were performed on days 7, 14 and 21 post burned. Results. At the day 7 of treatment, a significant statistic difference was observed for the burns area of the groups (p = 0,002). The area of wounds treated with HA-Q-CS was significantly lower than those treated with nitrofural (p = 0,001). On day 14 there was no significant statistic difference between groups (p = 0,063). On day 21 there was a significant statistic difference between treatments (p = 0,005), the skin treated with HA-Q-CS had an area significantly lower than the treated with furacin (p = 0,005); Also, the skin treated with ET hada significantly lower area than the skin treated with nitrofural (p =0,033). The major wounds retraction confirmed with the microscopy was obtained with HA-Q-CS at the day 7, 14 and 21 of treatment. Conclusion. HA-Q-CS showed a significantly major effect than the hdrialcoholic extract of C. spinosa alone, the chitosan-poly vinylalcohol (without C. spinosa) and the nitrofural.