Process for the production of macrolide compounds

Fermentation product A83543 (1), comprising major components A83543A and A83543D and minor components A83543B, A83543C, A83543E, A983543F, A83543G, A83543H and A83543J, is produced by a newly described species, Saccharopolyspora spinosa . The A83543 components and their acid-addition salts (A83543 compounds) are useful as insecticides, particularly against Lepidoptera and Diptera species. Insecticidal, miticidal or ecto­parasiticidal combinations, compositions and methods are provided. wherein R is H or a group selected from R² is R¹, R³, R⁵ and R⁶ are hydrogen or methyl; R₄ is methyl or ethyl; or an acid addition salt thereof the compounds when R is other than hydrogen.