Enzyme-labeled antigen for protein anabolic hormone immunoassay and analysis method

The invention provides an enzyme-labeled antigen for protein anabolic hormone immunoassay and an analysis method and belongs to the immunochemical analysis technology. The enzyme-labeled antigen for protein anabolic hormone immunoassay is prepared by connecting nandrolone hapten and horseradish peroxidase, and the nandrolone hapten is prepared by introducing carboxyl on the basis of hydroxide radical of nandrolone. The enzyme-labeled antigen can be applied to protein anabolic hormone multi-residue chemiluminescence immunoassays of nandrolone, trenbolone, propionic acid nandrolone, Stanolone and mestanolone. The analysis method overcomes the defect of low sensitivity of the traditional Enzyme linked immunosorbent analysis method and improves the accuracy and detection efficiency of immunodetection.