Optimization Of Medium Components For Reactive Orange M2r Dye Degradation By Consortium Vss Using Response Surface Methodology

Discharge of wastewater from textile dyeing industries has been a problem in terms of pollution and treatment of these waters is a great task. Keeping this in mind, the aim of this current research is to study the effect of various bioprocess variables on decolorization of an azo dye, Reactive orange M2R (ROM2R) by a bacterial consortium VSS. Response surface methodology was used to optimize the important physical parameters screened by Placket-Burman design. 12 Physical parameters such as Glucose(%), Starch(%), Sucrose(%), Yeast extract(%), Peptone(%), Ammonium nitrate(%), pH, temperature(0C), dye concentration(ppm), inoculum size (v/v) and time (h) were tested by using Placket-Burman design criterion and 4 parameters out of 12 (Yeast extract, pH, Temperature, Inoculum size) showed significant effect (P<0.05)) on decolorization of ROM2R using consortium VSS. The values of parameters were optimized by applying central composite design (CCD) and the most suitable values for ROM2R decolorization by consortium VSS, as predicted by the statistical tool, was Yeast Extract 0.3%, pH 6; temperature 35 0C, inoculum size (v/v) (%) 12.5%. At these optimum levels of parameters, bacterial decolorization of ROM2R by 95% was obtained under static conditions.