Method for extracting lipid from Chlorella sorokiniana CS-01

The invention discloses a method for extracting lipid from Chlorella sorokiniana CS-01 (CCTCC M209220), namely an ultrasonic-assisted chloroform/methanol extraction method. The method mainly comprises the following steps of: collecting algae, drying, extracting by using chloroform/methanol, performing ultrasonic crushing, washing, and drying. Through the certification, compared with a Soxhlet extraction method, an acid heat extraction method, and a chloroform/methanol extraction method, the method has the advantages that: the extraction ratio of the lipid is improved by 1.4, 1.7 and 0.5 timesrespectively; meanwhile, a lipid extraction phase is positioned under a cell debris solid phase, so the method is favorable for the subsequent direct separation of the lipid, and meets the requirements of mass industrialized production.