Электрическая проводимость эмбрионов мышей доимплантационных стадий развития после гормональной стимуляции яичников животных

Ovary stimulation in mammals with usage of gonadotropic hormones is an integral stage of assisted reproductive biotechnologies. At the same time it is known that hormonal stimulation causes the ovulation of gametes of different ability to develop. Necessity to investigate the dynamics of gamete and embryo functional state change stipulates the search for additional cell parameters and methodical approaches to their determination. Specific electric conductivity of one-, two-, and eight-cell mouse embryos obtained following hormonal stimulation has been determined by the method of impulse conductometry. It has been shown that the values of electric conductivity are gradually changed after fertilization as well as during an embryo clea vage. The distribution character of this electric parameter becomes more homogeneous. The range of the values of electric conductivity of mouse zygotes has been shown to be (2,16±0,24÷7,38±0,58)⋅10 –3 S/m. The ranges of values of electric conductivity of twoand eight-cell mouse embryos have been shown to be 1,54±0,11÷6,12±0,34)⋅10 –3 S/m and (1,21±0,15÷5,55±0,26)⋅10 –3 S/m, correspondingly. The investigations indicate that an electrical conductivity is a sensitive parameter, which allows detecting the hidden disorders of embryo functional state and studying the dynamics of its change. The state of nuclear material of embryos with morphological injury has been investigated by Hoechst-staining method. On the base of the obtained and literature data probable mechanisms of apoptosis in early mouse embryos are discussed.