[Expressions of NF-κBp65 and IκBα in gestational trophoblastic disease and clinical significance].

AIM: To investigate the roles of nuclear factor κB p65(NF-κBp65) and inhibitor of nuclear factor κB α(IκBα) in the development and metastasis of gestational trophoblastic neoplasia(GTN) by analyzing the expressions of NF-κBp65 and IκBα in normal early pregnancy villi and gestational trophoblastic diseases,and to reveal the relationship of NF-κBp65 and IκBα with age and clinical stage of GTN patients.METHODS: The expressions of NF-κBp65 and IκBα were detected by immunohistochemistry in normal pregnancy villi(20 cases),hydatidiform moles(HM,30 cases),invasive hydatidiform moles(IHM,13 cases) and chorionic carcinomas(CCA,15 cases).RESULTS: NF-κBp65 expression was statistically different(P0.05) between normal pregnancy villi and IHM(P=0.013),normal pregnancy villi and CCA(P=0.018),HM and IHM(P=0.026),HM and CCA(P=0.035).Differences in IκBα expression were statistically significant between normal pregnancy villi and IHM,normal pregnancy villi and CCA,HM and IHM,HM and CCA(P0.01).The expressions of NF-κBp65 and IκBα were correlated to clinical stage(P=0.043,0.042,P0.05),but not to patients' ages.Spearman correlation analysis revealed that there was a negative association between the protein expressions of NF-κBp65 and IκBα in GTN(r=-0.403,P=0.034,P0.05).CONCLUSION: Up-regulated expression of NF-κBp65 and down-regulated expression of IκBα may be related to the development,invasion and metastasis of GTN.The expressions of NF-κBp65 and IκBα are negatively correlated in gestational trophoblastic tumor tissues.