The Effect of Nano-Transferosomal Hydroxyurea on 4T1 animal Breast Cancer Cell Line: An in Vitro Study

Breast cancer considered as a major health problem in women worldwide. Despite the various chemotherapeutic strategies, complete and effective therapy with lowest side effects didn’t reach until now. So, in this regard, evaluation of the new treatments based on nanotechnology chemotherapeutic agent's drug delivery gained a much attention. The aim of the present study was to formulate, optimize and investigate the potential of novel nano-transferosomal hydroxyl urea (HU) effect on 4T1 mouse breast cancer cell line. Nano-transfersomal HU were prepared using reverse-phase evaporation method. Then evaluated for size, encapsulation& loading yield and drug release pattern. Cytotoxic effects of prepared Nano-drug in compared to free standard form evaluated by MTT assay. The optimized nano-transfersomes HU formulation showed vesicles size of 52.09±4.6 nm. Also, Encapsulation and loading yield were estimated 97.09±4.4 and 4.2±0.3 respectively. The optimized nano-transfersomes HU shows a higher cytotoxic effect in the dose dependent manner in compared to standard HU. The present study confirmed the higher cytotoxic effects of HU due to encapsulation (nano-transferosomal form). It was concluded that the nanotransfersomes provides an improved drug delivery in 4 T1 breast cancer cell line.