Rapid detection method of paraquat in patient blood

A rapid detection method of paraquat in patient blood comprises the following steps of 1) establishment of a paraquat high performance liquid chromatography (HPLC) method, wherein the HPLC conditions are that: a high performance liquid chromatograph is used, the chromatographic column is a shim-pack:vp-ODS column (4.6mm*250mm, 5 microns), the mobile phase is acetonitrile-0.02 mol/L sodium 1-octanesulfonat, and the pH value of the sodium 1-octanesulfonat is adjusted to 2.5 by using 0.26 mol/L phosphoric acid, the flow velocity is 1.0 mL.min , column temperature IS 25 DEG C, and the detection wavelength is 254 nm; 2) preparation of PQ drug-containing serum, wherein a control is prepared by precisely sucking 200 microliter of serum from a healthy person, adding 100 microliter of a PQ aqueous solution with a known concentration, adding 400 microliter of acetonitrile, mixing, centrifuging the suspension for 10 min, taking the supernatant, centrifuging the supernatant for 10 min, taking the supernatant to perform HPLC analysis; 3) preparation of a sample, which comprises precisely sucking 200 microliter of serum from a patient, adding 400 microliter of acetonitrile, mixing for 1 min, centrifuging the suspension for 10 min, taking the supernatant, centrifuging the supernatant for 10 min, taking the supernatant to perform HPLC analysis; 4) preparation of a blank solution, which comprises precisely sucking 200 microliter of serum from a healthy person, adding 100 microliter of distilled water, adding 400 microliter of acetonitrile, mixing for 1 min, centrifuging the suspension for 10 min, taking the supernatant, centrifuging the supernatant for 10 min, taking the supernatant to perform HPLC analysis.