Effects of low-dose γ-ray on the expression of CCNG1 gene in human lymphoblasts

Objective　To investigate the effects of low dose γ-ray on the gene expression of cyclin G1 (CCNG1) in human lymphoblasts AHH-1 line and the probability of CCNG1 as bio-dosimeter for low-dose radiation. Methods　Lymphoblasts of AHH-1 line were irradiated with γ-ray in the doses of 0, 0.1, 0.2, 0.5, 0.8 or 1.0 Gy. Total RNA of cells was extracted at 0, 4, 24, 48, 72 and 168h after irradiation, and real-time Q-PCR was used to detect the expression of CCNG1 gene in AHH-1 cells. The relationship between the irradiation dosage or duration and the gene expression was analyzed. Results　The gene expression of CCNG1 in AHH-1 cells increased in a dose-dependent manner after irradiation, presenting a better dose-effect relationship. Time-effect curve indicated that the peak expression of CCNG1 appeared at 24h after irradiation, and then the expression decreased gradually with time, and it recovered to the level before irradiation at 168h after irradiation. Conclusions　CCNG1 gene is sensitive to low dose irradiation (0-1.0Gy) with a better dose and time-effect relationship, and it may be used as a potential efficient biological dosimetry in low dose exposure risk assessment. DOI: 10.11855/j.issn.0577-7402.2015.06.16