USE OF ON-LINE OUR MEASUREMENTS TO MONITOR AND IMPROVE INSECT CELL CULTURES FOR RECOMBINANT PROTEIN PRODUCTION

On-line oxygen uptake rate measurements using a dynamic model are applied to Sf9 cultures infected with recombinant baculovirus. This measurement allows a sensitive monitoring of cell growth and post-infection process. Also, results are given on fed-batch and perfusion cultures were 8–10 ·106 cells/ml can be achieved by automatically adjusting the medium fed-rate on the basis of OUR on-line measurements.