The Determination of Magnolia liliiflora Pollen Viability and Its Storage Method

In order to explore appropriate storage method and optimum determination method and study the relationship between the pollen life and the storage environment,isolated culture,carmine acetate dyeing、I-KI staining and TTC staining methods were used to determine the fresh pollen ability of Magnolia liliiflora.The results showed that culture media with different concentration of sucrose and boric had good effects on the pollen germination.The pollen germinating percentage reached the highest(90.05%) after incubated on the media supplemented with 2% sucrose and 0.001% boric acid.The best conditions to keep vitality of pollen is in not dry environment at-80℃,the storage ability lasted for more than 5 d.