Inhibitory effect of tumor necrosis factor on gene expression of hormone sensitive lipase in 3T3-L1 adipocytes.

: Recombinant human tumor necrosis factor (TNF) depressed the activities of both lipoprotein lipase (LPL) and hormone sensitive lipase (HSL) in 3T3-L1 adipocytes, 3 to 24 h after its introduction to the cells. HSL gene expression, as measured by Northern blotting analysis with 32P-labeled cloned HSL-cDNA, was also suppressed. These results suggested that the reduction in HSL activity caused by TNF resulted from inhibited gene expression of the enzyme.