Состав мукозной микрофлоры желудочно-кишечного тракта при синдроме раздраженного кишечника

The aim of this study: to investigate and analyse the gastrointestinal mucosal-associated microbiota in the samples from diarrhea-predominant IBS (D-IBS), соnstipation predominant IBS (C-IBS) patients and healthy controls. Methods. Oral cavity, duodenal, colonic and rectal mucosal samples were obtained from 40 IBS patients (20 C-IBS, 20 D-IBS) and 14 healthy controls. Duodenal and colonic tissue was collected during a flexible duodenoscopy and colonoscopy. Tissue samples were frozen for further molecular analysis. DNA was extracted from all frozen samples and used to identify 29 specific bacterial groups using quantitative real-time PCR (qPCR). A statistical treatment of the received data was performed. Results. The dominant groups of bacteria of various microbiotops of the gastrointestinal tract in IBS patients and healthy controls were determined. qPCR analysis of duodenal samples demonstrated a reduction in the Bifidobacterium concentration in tissue samples from C-IBS patients when compared to healthy controls (р