Abstract 13856: Circulating microRNA Differentiates HFpEF and HFrEF Patients

Introduction: Circulating microRNAs (miRNAs) might reflect altered regulation of key pathways that determine the progression of heart failure (HF) and therefore provide therapeutic targets. We explored the feasibility of serum miRNA profiling in patients with HF and whether miRNA profiles are different in i) non-HF control vs. HF and ii) HF with preserved ejection fraction (HFpEF) vs HF with reduced ejection fraction (HFrEF). Methods: Serum samples from 124 subjects were obtained from non-HF controls (n=61), patients with HFpEF (n=51), and patients with HFrEF (n=12). miRNAs were isolated, and levels of 84 miRNAs known to exhibit altered expression in cardiovascular disease were assessed using real-time quantitative PCR. Data analyses were carried out using R limma package. Multiple testing was adjusted by Benjamini-Hochberg’s approach. Results: 57 out of 84 miRNAs had detectable expression in the serum (Ct mean<35), in which 3 miRNAs (miR-122, miR-142, and miR-143) differed significantly between HF and co...