Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis
2007
microfabricated capillary array electrophoresis (mu-cae) was applied to study the interaction between minor groove binder netropsin and a non-selfcomplementary 12 mer double stranded oligodeoxynucleotide: d(cccctataccgc) center dot d(gcggtatagggg). esi-ms was used to provide an independent verification of the microchip electrophoresis derived data. simultaneous parallel quantitative assay of multiple samples was performed in a single run (< 50 s) on the self-developed mu-cae device. the binding constant and stoichiometry calculated from scatchard plot were (2.88 +/- 0.23) x 10(5) m-1 and 1:1, respectively. the values showed a good quantitative agreement with the results determined by esi-ms and those using other methods reported in the literature.
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