Production of a Mycobacterium avium ssp. paratuberculosis purified protein derivative (PPD) and evaluation of potency in guinea pigs

Abstract A Mycobacterium avium ssp. paratuberculosis purified protein derivative (PPD) was produced and the biologic activity evaluated in sensitized guinea pigs. The PPD when adjusted to a protein concentration of 1 mg/ml induced a delayed-type hypersensitivity response comparable to USDA Johnin OT 133-8707. Copyright 2002 The International Association for Biologicals. Published by Elsevier Science Ltd. All rights reserved .