HPLC를 이용한 당류의 동시분석법

In order to develop a good separation and simultaneous analysis of different sugar in an artificial mixed sugar solution, we analyzed 10 sugar components in an artificial mixed sugar solution composed of fructose, glucose, mannitol, sucrose, maltose, lactose, xylose, xylitol, erythritol, and trehalose with using HPLC-ELSD or HPLCRI. Separation and Quantification by I-IPLC-ELSL) was superior to those by 1-IPLC-R1 and detection sensitivity by HPLC-ELSD was higher then that by HPLC-RI as microgram(Ltg) level. 1. The units of minimal detectable limits were showed pg/me and ng/m by the HPLC-RI and I-IFLC-ELSD, respectively. 2. The condition of ELSD was drift tube temperature 82 C, N2 gas flow rate 2.10 SLPM, and column oven temperature 30 `C, respectively. Isolation and recovery rates of single sugar from the multiple sugar solution was higher at the condition (time: flow rate: D.W.: ACN MeOR, mm: ni/min: v: v: v) of linear gradient elution of mobile phase as 0: 1.00:15: 85: 0,1:1.00:6:90:4, 17:1.00:10:70:20, 23:1.00:15:85:0 and 35: 1.00:15:85:0, in order.