CAPILLARY ZONE ELECTROPHORETIC SEPARATION OF PROTEINS USING COATED CAPILLARIES

ABSTRACT The possibilities of capillary electrophoresis for the analysis of several basic and acid proteins by using different capillary modification procedures are shown. In order to reduce the interaction of proteins onto the capillary inner wall, potassium chloride (KCl), polyethyene glycol (PEG), poly(vinyl alcohol) (PVA), and hydroxyethylcellulose (HEC) were tested as additives in the running buffer. Furthermore, a PVA coated capillary was examined to compare the suitability of coating to separate different nature proteins (lysozyme, carbonic anhydrase, trypsin inhibitor, and bovine serum albumin) by capillary electrophoresis.  The experiments were carried out at different concentrations of the additive, voltages and, in some cases, pH. This study showed that the PVA provides the better results, since both kinds of proteins could be analysed simultaneously without important adsorption drawbacks. When a poly(vinyl alcohol) coated capillary was used, the improved performance over bare fused-silica capi...