Paralytic Shellfish Poisoning on French Mediterranean coast in the autumn of 1998: as a causative agent Alexandrium tamarense

Analysis of the main PSP toxins was performed using the post-column oxidative fluorescence method of Oshima et al. (1995) with slight modifications. Toxins were separated by reversed phase chromatography using a C8-silica column (5 μm Develosil, 4.6 mm i.d. x 250 mm) with a flow rate of 0.8 ml/min. Toxins were quantified in duplicate with certified toxin standards (PSP-1B) provided by the NRC (Institute for Marine Biosciences, Halifax, Canada). Two Gonyautoxins (B1, B2) and C-toxins were detected and quantified using acid hydrolysis (HCl 0.4N, 97°C, 5 min.) as indicated by Franco & Fernandez 1993. Mytilus edulis galloprovincialis Crassostrea gigas Ruditapes decussatus