普罗布考在抗ox-LDL诱导THP-1巨噬细胞凋亡中对CD36、Caveolin-1表达的影响

Aim To study the effects of probucol on THP-1 macrophage apoptosis and CD36、Caveolin-1 expression induced by ox-LDL. Methods Apoptosis of THP-1 macrophages was determined by flow cytometry analysis. RT-PCR and immunofluorescence were used to detect CD36, Caveolin-1 mRNA level and protein expression respectively. Results Probucol had no effect on mRNA level of CD36, Caveolin-1 in THP-1 macrophages, but it attenuated Caveolin-1 protein expression. Conclusions Probucol can inhibit apoptosis induced by ox-LDL in THP-1 macrophages by down- regulating Caveolin-1 protein expression.