[137-POS]: MTHFR and COMT possible epistatic and epigenetic interactions and its modulation of some cardiovascular risk parameters in women with previous pregnancy hypertension.

Objectives To study the MTHFR and COMT polymorphisms and its interactions as susceptibility factors for the development of PE, as well as, to study in a follow up group, its influence on some biomarkers of cardiovascular risk at long term. Methods A sample of 327 women (28.85  ±  5.59 years old), with 142 controls (normal blood pressure in pregnancy, NBPP) and 185 with preeclampsia (PE). We also performed a prospective study in a sub-sample of 138 women (35.24  ±  5.48 years old), 90 of these presented previous PE, 2–16 years ago. The MTHFR and COMT functional polymorphisms were evaluated by PCR-RFLP. We evaluated demographic, anthropometric, hemodynamic and other parameters: C-reactive protein (CRP), liver function (AST, ALT and GGT) and lipid profile (cholesterol total LDL, HDL, non-HDL, Apo A and B) which were determined by conventional methods. Results The MTHFR CC+CT genotypes were associated with risk for the development of PE (OR = 4.175, 95% IC 1.361–12.806, p  = 0.015). Previously PE women, with these risk genotypes, presented significant differences in some risk parameters (BMI, WC, SBP, DBP, Pulse Pressure and Apo B) ( p p  = 0.008). In the prospective study, considering the MTHFR CC+CT genotypes in previously PE women, some parameters (BMI, waist, SBP, DBP, PP and Apo B, p Conclusions The distributions of MTHFR CC+CT genotypes were associated with the development and susceptibility of PE. These risk genotypes may modulate the history of PE leading to an increase of some risk cardiovascular parameters, even in women previously PE that became normotensive years after pregnancy. A. Matos; None. A. Pereira da Silva: None. H. Maia: None. M. Clara Bicho: None. I. Rebelo: c.None. M. Jose Areias: None. M. Bicho: None.